Tag: embryo transfer

Final Cycle – Day 29

Final Cycle – Day 29

11dp5dt or 16dpo

Today is the day i wanted to test originally so at 6.30am i once again pee’d on a stick

Definitley pregnant!!! 

This is the comparison photo from Saturday night to this morning

Tomorrow is actually Official Test Day where i will have bloods done and get a figure for the amount of Hcg in my system (thats the pregnancy hormone) . Not that the number will tell us anything other than im pregnant.

With Millie my hcg was 814, a friend of mine had her result come in at 130 something and she’s having twins, so numbers mean nothing. Itll be fun to compare and speculate however.

My early scan is booked for when i am 6 weeks and 4 days, today i am 4 weeks and 2 days.

I will be honest, as always, if more than 2 embryos have implanted, i will be opting for foetal reduction. Ill cross that bridge if we come to it and discuss my reasoning for this at that time.

Its really not like me to test early but over the course of Saturday, i was tying myself up in knots, going round and round in my head thinking it had and hadnt worked. Trying to deal with emotions i had no idea if i needed to deal with. In short it was tearing me apart and Mr Me couldnt bear to see me like that so eventually told me to do it so we knew what we were dealing with.

The 2 week wait is always tough but it REALLY got to me this time round. Possibly because i knew it was my last chance or just because 6 previous 2 week waits really dont prepare you for another. Who knows? It all worked out in the end.

Im still finding it a bit surreal, its not sunk in yet still. Im calm and positive though. The ‘fear’ hasnt hit me i.e. will we make it to term, will i have to terminate again, am i going to lose this pregnancy? 

Im just soooooo pleased that cycle 9 worked. Im so happy i am ABLE to get pregnant again as i was doubting it. Im excited for my bump, to wear maternity clothes, to feel wonderfully well and like im special and part of a secret club.

Love, Little Miss PMA xxx

Final Cycle – Day 20

Final Cycle – Day 20

Today i am 2 days past a 5 day transfer (2dp5dt) or 7 days post ovulation in a tradtional cycle.

Ive been very lazy as per clinic instructions. Had a very nice roast chicken dinner and a lovely nap just now.

Had another dizzy spell and more cramping which im taking as a good sign seeing as i felt nothing in June! 

One of my fur babies needs a trip to the vets but as he is heavy, Mr Me is going to take him tomorrow so i dont have to lift him! 

Above is an animation of all my embryoscope pictures. Its a bit fast but pretty awesome. Ill also post a copy of my DVD once ive watched it. But im just about to watch Captain America: Civil War instead so itll be tomorrow now! 😂

I have a positive feeling about this cycle so far although im struggling between thinking it might just actually work and being afraid to get my hopes up. 

Hopefully over the coming days ill become braver and even more positive.

As hot as Cyprus was, the UK seems freezing, got my big fluffy dressing gown on already! 

I mentioned Mr Me’s blue hair in my last post…

Truly awesome in my humble opinion.

Have a good evening folks

Love, Little Miss PMA xxx

Final Cycle – Day 19

Final Cycle – Day 19

Home time!! 

Yesterday was our final day in Cyprus. It was a whopping 38°C and so i stayed inside. Even so, i struggled with the heat and had a slight dizzy spell.

So i drank plenty and kept a cold flannel on my neck. I did feel better after a shower too. My Mum managed a trip to the pool and was fine. Its unusual for me to struggle in the heat.

Ive been ‘aware’ of my uterus since transfer. Sometimes it feels quite tight/crampy and i get the occassional sharp pain in one specific spot.

I think my ovaries are still quite large and i swear the top is still sore from being punctured during Egg Collection, although yesterday was tbe 1st time i felt it???!!! 

So yeah, it looks like im symptom spotting! 

I have to say im feeling alot more than i did after my transfer in June and its keeping me positive. Right now im quietly confident but its such early days im scared to get my hopes up.

Ill be glad to get back to work for distraction.

My lovely Mum insisted i lift NOTHING during our return to the UK which was difficult to watch when she is in so much pain. However, mostly people are kind so between the villa manager, taxi driver and wheelchair assistant guys at both airports, i didnt lift a thing other than hand luggage! 

It was wonderful to see Mr Me again last night at 2 in the morning. He had a hat on though and covered his blue hair so took me a minute to spot him. My eyesight can be terrible!!! 

We are not ones for PDA, especially when itd be holding up others but we were just so happy to be back together!!! 

We came home and crashed. Later my brother is coming over and we are having Sunday lunch before he takes Mum home. Itll be quite emosh when she goes as shes been my little cheerleader.

I met some amazing people in Cyprus, and im honoured to be part of their journey. It was lovely to be able to share my knowledge and offer reassurance. I should look into this as a career 🤔

Love, Little Miss PMA xxx

Final Cycle – Day 18

Final Cycle – Day 18

Embryo Transfer!!! 

Id been asked to come to clinic for 10.30am so we were up early and started the water-thon to fill my bladder. We arrived a little early so i could keep drinking, Mum doesnt drive so the half an hour journey was liquidless and i wanted to be sure i had a full bladder as in a previous transfer it wasnt full enough.

I needn’t have worried, in the end i went a bit mad and totally overfilled my bladder. 2.5ltrs is FAR too much and VERY uncomfortable.

Anyway, upon arrival i was shown to my room (i was still drinking at this point) and Umit explained that we had 6 not 7 healthy embies (just whatsapped me the wrong number is all) but as im not after 6 babies, its allllll good.

The scientist in me wanted to know which embies were unhealthy and what they had.

Number 4 in the embryoscope had trisomys 13 & 18 (Patau & Edwards Syndromes)

Number 7 in embryoscope had Turners Syndrome. 

Im really very pleased we had PGD as without it, those 2 embies could well have been transferred.

Umit also told me that number 1 (originally in table top incubator and now known as ‘table top’) was beginning to hatch!!!! We have never ever had a hatching blastocyst before. It made me begin to believe this could work.

Dr F had a couple of collections to do and a scan appointment so i was left to my own devices for a bit. At this point i knew i had drank far too much! 

I did one ‘little wee’ to help with the pressure, but by the time i was collected for ET (embryo transfer) i’d had to do another. Its very hard not to fully pee when you are THAT full let me tell you! 

So im wheeled to theatre, given my hair net and get myself on the table. The nurse does an ultrasound and after a few minutes declares im too full and need to wee a bit more. Cue me waddaling around in an open backed theatre gown, hilarious.

Anyway, after my 3rd ‘mini wee’ im back on the table and the image is clear.

Lovely Dr F comes in and starts to prep me and tells me ‘i want you pregnant this time Toni’. You and me both lovely! 

The image was so good that i saw the cathether enter my uterus and i kept my eye on it. After the lights are dimmed, Mr Embryologist brings in my 4 (2 hatching, 2 blastocysts) babies and Dr F pushes them through. And there on the screen, a white flash as they entered my uterus!!! Eeeep!!! 

Once again Dr F tried to give me a catheter to relieve my VERY full bladder but alas it was too big, again. Instead she gave me a lovely pep talk about my chances, i thought i might pee on her! 

So ET was at 12.20pm, wheeled back to my room and told i had to wait 30 mins before i could have a bed pan. Longest. 30. Minutes. Of. My. Life!!!! 

I lasted 25…..ohhhhh the relief. No embarrassment this time re the bedpan, just pure joy!!! 

After my lengthy pee, the nurse did my clexane and proluton depot and i was left to my 2hr lie down and a meal.

Halfway through my meal i had to pee again. Cue poor Mum getting the bed pan out for me again!!!! 

I felt some light cramping whilst lying down….possibly because, number 12 was also hatching by the time it went back.

These are the final embryoscope images…

The colour coding shows what was good for transfer and what needed disregarding. Embryoscope is the bomb.

Close ups of the transferred blastocysts below

‘Table Top’ almost fully hatched blasto
Number 6 -Blastocyst
Number 11 – Blastocyst
Number 12 – partially hatched Blastocyst

Ive spent hours looking at these pictures. Ive never had 1, let alone 2, hatching blasts its just incredible.

After clinic, we had cake, Umit put it into our heads. We got home about 4, put a wash on and promptly fell asleep! 

Its been an emosh day! I couldnt be happier. If this doesnt work, it never will.

Let the 2 week wait begin. Crumbs.

Love, Little Miss PMA xxx

Final Cycle – Day 17

Final Cycle – Day 17

Embryo development day 4.

This mornings embryoscope looked a little something like this (well, exactly like this) 

As the clinic said, they look like beaitiful flowers! Being biopsied didnt do any harm as they are all developing nicely.

We’ve had a lovely day, playing in the sea, and then meeting some lovely people for coffee (again!). Its my last chance as tomorrow is embryo transfer day! 

Whilst at coffee i was having a good ol’ chin wag with some fellow patients, little did i know the team were trying to contact me! 

Why? Well, they had some very exciting news for us…

Of the 8 that were sent for testing, a whopping 7, yes 7, are healthy!!!!! I was literally stunned into silence. If you know me, youll know thats pretty damn hard to do!!!! 

Its still not sunk in if im honest. On average 50% come back unhealthy so i was hoping for 3 or 4, but 7???? Nope, never even imagined it. Still cant believe it, and ive known 5 hours!!!! 

Im literally the happiest girl alive as it means the wonderful Dr Firdevs has her pick tomorrow of which ones to put back based on embryo development. 

Never did i dream id get this lucky. Never. Im so so grateful to Team Miracle.

A little part of me is daring to dream we may end up pregnant…..shhhh! Dont tell the take home baby gods though.

Im still going to take one day at a time and see what happens, but oooosh, couldnt be more pleased with the results.

Im at clinic 10.30am local time so think of me as you head off to work UK followers, i may be having my take home baby transferred.

Toodle Pip

Love, Little Miss PMA xxx

IVF – a basic guide

IVF – a basic guide

IVF is a complex process that takes months to set up and then weeks/months to execute. If you are new to IVF its very daunting as there seems so much to get your head around and understand. So ive put this together for my non ivf friends and those just starting out.

Easiest way to do this is a timeline:

Within 6 months of starting treatment youll need a number of tests performed on days 2-4 of your menstrual cycle. These tests include:

  • AMH – this looks at your egg reserve
  • FSH
  • LH
  • Oestriodal
  • Prolactin
  • Thyroid stimulating hormone

Youll also need a scan in the same time frame to check you antral follicle count (AFC) this is simply the number of follicles on each ovary before treatment. This number is NOT the number of eggs you will get at collection.

Some clinics also run STI blood tests for HIV and Hep A, B, et all aswell.

The good thing about these results is there is no bad or good. They are simply to help the Doctor prescribe the right stimulation drugs. Do not read too much into these results, you may be above or below average but thats not what is important, what is important is that Dr has the most current info possible in order to stimulate safely.

Its worth noting that many UK clinics see the 1st attempt as a trial run to see how your body responds (even with all the test results Drs have no clue how you as an individual will react) and that is why many 1st cycles fail and drugs are tweeked for the 2nd cycle.

So, now we have all the tests done, what happens next? Well your clinic needs to fit you in. This usually involves calling on day 1 of your cycle and awaiting a call back for availability (and this is why i dont cycle in the UK – the waiting lists are absurd at some clinics) 

Once accepted, youll be given a drug teach appointment. If cycling abroad you dont get this but there are loads of youtube videos that you can watch!! 

Ive only ever done short protocol on a fresh cycle (fresh means eggs are collected and replaced in same cycle) so im going to focus on short protocol.

Short Protocol

Period starts = day 1. If your period arrives after midday the NEXT day is CD1.

Day 2 = stimulation medications start (usually gonal f or menopur)

Days 3 – 6 = stimulation meds

Day 7 = add in medication to STOP ovulation (dont want all those eggs being let loose) and its usually Cetrotide.

In the UK you will be monitored via bloods and scans but nothing really starts happening until the 2nd week.

The scans are to look at FOLLICLE development…they will be counted and measured. Usually they need to be around 18mm to be considered ready for collection. During this time, your stimulation meds can be adjusted accordingly during monitoring.

Once the follicles are ready youll be given a trigger or hatching shot. It basically tells your body its ok to ovulate in the next 36hrs. When you take the trigger Egg Collection will be EXACTLY 36 hours later.

The day after trigger is a DRUG FREE day, woohoo!!! Its the only one in the cycle mind, but its welcome as youll be quite battered and bruised.

Egg Collection

This is a medical procedure and the majority of clinics sedate you meaning nil by mouth from midnight the night before. Its light sedation but youll not remember the procedure which is nice…plus the after affects are funny (see ‘Time for a laugh’ for details)

During the procedure the Dr will aspirate your ovaries. Remember not every follicle will contain an egg.

After collection IVF/ICSI or IMSI will be performed

IVF – the sperm and eggs are placed in a petri dish and left overnight to fertilise

ICSI – used when sperm count is low or of low quality. An embryogist looks through a microscope, physically selects the best looking sperm and injects it into an egg (its actually a lot more complex than thar but unless you are a science geek like me, its probably not of interest) 

IMSI – very similar to ICSI but with a stronger lens in the microscope i believe. 

Embryo Development

Over the coming days the embryos will be placed in an incubator and cultured in a medium. The embryologists check the embryos daily to monitor cell division and growth. During this time some embryos are likely to arrescyc

During this time you will be prepping your body for transfer. You will most definitely be given pessaries, waxy bullets containing progesterone that is inserted vaginally or anally. You may also be given estrogen support to thicken your lining, antibiotics, steroids etc. All clinic and protocol dependent. 

Embryo Transfer 

This can take place 2, 3 or 5 days after collection. It all depends on the number of embryos and the quality. Only with high numbers will most clinics go to day 5 development. There isnt a best day for transfer. Pregnancies can happen from any day transfer 

It is thought that by day 5 only the strongest will have survived when there is a high number of embryos making selection for transfer easier

Embryo Transfer

Ita basically a smear with a full bladder. Uncomfy but not painful. The full bladder is for the sonagram thats performed during the procedure. It allows the Dr to see inside your uterus and place your embryos in the best spot. If youre lucky youll see a flash on the screen as your embryos are transferred by catheter. And thats it. You are pregnany until proven otherwise! On a short protocol the time taken matches your menstrual cycle so its all done in 4 weeks and that includes the 2 week wait.

2 week wait

I wont lie, its mental torture. You analyse every twinge, sneeze, cramp wondering what it means. There is no cure, just keep as distracted as possible until test day. Im not an early tester and would recommend you dont test until 9dp5dt (9 days past a 5 day transfer) or 11dp3dt or 12dp2dt as then the result will be accurate. I usually break at 11dp5dt as my clinic’s OTD (Official Test Date) is 12dp5dt.

Fingers crossed youll get a positive result but remember IVF isnt a gurantee, even if youve had children tradtionally before. Even when everything is text book, once transferred its all in natures hands and it can still fail (which has happened to me more than once) 

Embryo Grading

MEANS NOTHING!!! Its something the embryologists use to classify embryos but a high grade embryo doesnt mean a baby, nor does low grade mean a negative result. Its just luck at that point to be honest.

Ive only outlined a basic ivf cycle, these days there are loads of extras you can opt for and ill discuss the ones im going for in more detail in future blogs.

I hope that helps give an idea of whats involved but feel free to ask me specifics and ill do my best to answer them. Also feel free to share with family and friends so they have an idea if whats happening when and then hopefully they wont ask lots of questions whilst you cycle.

Love, Little Miss PMA xxx

Different isnt bad…

Different isnt bad…

Im well pissed off at myself. I’ve let these negative feelings basically ruin my PUPO bubble. This is NOT like me.

Ok so this cycle is different, well actually better, grade AA embies, thickest lining etc so why am i so negative??

Im symptom spotting thats why. And thats just stupid.

Hcg is only just being released so why would i feel different??? Its TOO EARLY!!!

After a few pep talks from friends, family and the clinic (how awesome are they?!) I am no longer going to be negative.

I hate being negative. Just because its different doesn’t mean its bad. Different can be good.

I now choose to believe that different is good, as in this is my take home baby.

Im so annoyed at myself for letting the crap get to me, i wouldn’t let any one else feel this way so i damn well wont let myself feel this way.

Negativity out, positivity in.

Screw you 2 week wait, im better than you.

Love, Little Miss PMA xxx

1dp5dt (1 day past 5 day transfer)

1dp5dt (1 day past 5 day transfer)

So the 2 week wait begins, or in my case, 12 day wait!

Today the blastocysts (stage my embryos were at when transferred) should be hatching out of their shell, ready to implant over the coming days.

I have had no symptoms to speak of, far too early, although i have felt a couple of tugging sensations this afternoon which ill take as a good sign!

Its been another gorgeous day and we have spent it in the shade to keep me cool.

I’ve also done some washing, rock n roll!!!

Highlight of the day…the power cut! Told mum we had no power, she turns on the kettle and asks me why its not working…

Me: “We have no power Mum so the kettle wont work!”

Mum: “oh you are an intelligent creature”

She genuinely meant it too…gotta love her 😂😂

Love, Little Miss PMA xxx

Im PUPO again!!

Im PUPO again!!

The day arrived! It was an early start after a late night, so not much talking was going on. We arrived a little early, as is always my way.

By this time i was wide awake and very excited. Also i had a VERY full bladder, as confirmed by ultrasound. Then i changed into my fetching theatre outfit and was wheeled to theatre.

Being short, getting my legs into those stirrups whilst also being low enough down the table, is a challenge, but once i was strapped in we were ready to go. My favourite nurse was on hand to assist Dr F during the transfer, but being a Sunday and with only 3 patients, it was a minimum staff.

Although, i do think everyone on shift was in that room with me at some point!!! Except the coordinator! But thats IVF, and you learn pretty quickly not to worry about being on show!

Speculum in and a little look around for my cervix, it likes to hide, and then the embryologist was called to bring through the catheter with my embryos.

And in they went….4 grade AA embryos are currently back where they belong, and im over the moon!!

Dr F tried to give my bladder some relief but i was too small or it was too big. I had to hold it.

Wheeled back to my room and Mum was surprised it was all over! Its as quick as a smear really!

A little later my nurse stabbed me with Clexane and Proluton Depot (i say stab, i mean administered in a totally professional manner 😂), blood thinner and progesterone in oil. The proluton is a massive one into the muscle and its pretty sore, even now. But i only need to have 6 over the next 20 days and its all worth it!

And now back to my bladder! I didn’t want to use the bed pan…i just don’t get it, peeing whilst flat but rightly so my nurse explained im meant to be stress free and a very full bladder is stressful.

Bed pan it was 😨😨

Peeing whilst flat is like going against nature, its just not what your body wants to do!!! But with loads of concentrating and telling my bladder its ok, sweet relief 😂😂

After my 2hr lie down with bum raised and legs up, i was allowed up.

The coordinator had been through my protocol and do’s and dont’s and got me my post transfer meds…its a lot!! I paid up and off Mum and I went.

We needed some shopping and Mum didn’t allow me to lift anything…cotton wool has been wrapped round me already!

Back to the villa for lunch and we have both had a nap following our early get up….lush!!

So here we go, in 12 days time we find out if it worked. It will. And in another couple of weeks, how many have implanted.

Seriously exciting times and yet again im so grateful to TM and Dr F for all they have done for me.

Im pregnant, until proven otherwise and i love it. You’ll not get me to test early as i like to keep the dream alive for as long as possible and I’ve seen far too many negative tests in my life to want to see more.

Wish me luck

Love, Little Miss PMA xxx

1 more day until transfer!!!!

1 more day until transfer!!!!

We’ve had a lovely day, relaxing by the pool BUT in the shade!! It was mighty warm again today!

Coming off the antibiotics has certainly stopped the nausea and i was able to have breakfast today…😂😂

Mid afternoon i was told the plans for tomorrow…
I’ve to be at clinic at 8.30am (Mum and I aren’t morning people so she is threatening to sleep in her clothes and make up 😂)
I need to be filling my bladder before and on the way and then some more when i get there!

I cant use a strong shower gel or deodorant….but its boiling so my plan is to have a shower before bed and put my deo on then so the smell is gone by morning…pretty smart i reckon?!

The very good news is, i CAN wear make up, which is useful as I’d scare the staff and patients without it…think zombie/vampire!!!

Im absolutely thrilled with how things are going and im so excited for transfer tomorrow….not something i would have said the beginning of the year.

Im so ready for this now. Im ready to be pregnant again, ready to deal with the anxiety the pregnancy will bring but most of all im so ready to be a Mummy!!! And Mr Me is so ready to be a Daddy!! Its all we’ve wanted for the last 7 years.

Im excited to ‘join in’ too, the freeze all did leave me feeling left behind, so it’ll be nice to feel im part of the club from tomorrow.

I cannot thank you all enough for all the support you’ve given me to get to this point. Family, friends, colleagues, and followers all of you have played a part in getting me to this point and ill never forget that.

To Mr Me…thank you for never giving up on me, even when i was spiralling out of control, for always being there, for knowing what to say, what to do and just being you. I cannot wait to start this new journey with you and i love and miss you sooooo much xxxx

Enough of being soppy….ill be PUPO tomorrow (see previous posts for meaning!)

Love, Little Miss PMA xxx